| General summary |
ONTRapid |
ONTLIG |
ONTLIGnoFrag |
PacBioRSII |
PacBioCLR |
PacBioHiFi |
| Mean read length |
9,231.4 |
11,597.7 |
10,410.0 |
17,529.9 |
18,904.2 |
12,924.1 |
| Mean read quality |
10.0 |
9.6 |
7.2 |
5.3 |
0.0 |
31.1 |
| Median read length |
4,253.5 |
5,935.0 |
1,661.0 |
17,759.0 |
13,942.0 |
12,883.0 |
| Median read quality |
10.2 |
9.9 |
7.2 |
6.1 |
0.0 |
30.7 |
| Number of reads |
31,032.0 |
1,370,414.0 |
97,301.0 |
163,478.0 |
3,383,930.0 |
1,789,131.0 |
| Read length N50 |
20,333.0 |
21,673.0 |
46,854.0 |
24,418.0 |
33,061.0 |
12,949.0 |
| Total bases |
286,467,413.0 |
15,893,590,553.0 |
1,012,898,671.0 |
2,865,756,312.0 |
63,970,329,624.0 |
23,122,913,014.0 |
| Number, percentage and megabases of reads above quality cutoffs |
|
|
|
|
|
|
| >Q5 |
31032 (100.0%) 286.5Mb |
1370414 (100.0%) 15893.6Mb |
97301 (100.0%) 1012.9Mb |
117926 (72.1%) 2637.3Mb |
0 (0.0%) 0.0Mb |
1789131 (100.0%) 23122.9Mb |
| >Q7 |
31032 (100.0%) 286.5Mb |
1308003 (95.4%) 15220.6Mb |
60867 (62.6%) 688.1Mb |
32013 (19.6%) 685.2Mb |
0 (0.0%) 0.0Mb |
1789131 (100.0%) 23122.9Mb |
| >Q10 |
17914 (57.7%) 185.8Mb |
624560 (45.6%) 7246.5Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
1789131 (100.0%) 23122.9Mb |
| >Q12 |
154 (0.5%) 1.0Mb |
531 (0.0%) 1.5Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
1789131 (100.0%) 23122.9Mb |
| >Q15 |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
1789131 (100.0%) 23122.9Mb |
| Top 5 highest mean basecall quality scores and their read lengths |
|
|
|
|
|
|
| 1 |
13.2 (808) |
13.7 (251) |
9.7 (290) |
9.4 (8774) |
-0.0 (4410) |
93.0 (1427) |
| 2 |
13.1 (198) |
13.6 (201) |
9.3 (378) |
9.3 (13660) |
-0.0 (24066) |
93.0 (1700) |
| 3 |
12.9 (291) |
13.4 (225) |
9.3 (226) |
9.3 (8079) |
-0.0 (20326) |
93.0 (1674) |
| 4 |
12.8 (6465) |
13.0 (213) |
9.2 (484) |
9.3 (13378) |
-0.0 (28238) |
93.0 (90) |
| 5 |
12.8 (1413) |
13.0 (135) |
9.2 (1207) |
9.3 (9974) |
-0.0 (12767) |
93.0 (4193) |
| Top 5 longest reads and their mean basecall quality score |
|
|
|
|
|
|
| 1 |
206567 (11.3) |
211292 (6.9) |
471734 (7.4) |
59304 (6.0) |
151906 (-0.0) |
26294 (32.7) |
| 2 |
205035 (10.0) |
203278 (10.6) |
292728 (7.0) |
57233 (5.9) |
141984 (-0.0) |
24594 (23.2) |
| 3 |
193875 (10.8) |
202734 (7.4) |
261182 (7.2) |
56444 (5.3) |
134001 (-0.0) |
23388 (23.9) |
| 4 |
181100 (10.2) |
200413 (7.0) |
258153 (6.9) |
56194 (5.0) |
127292 (-0.0) |
23092 (24.9) |
| 5 |
177906 (10.9) |
189824 (6.6) |
255887 (7.0) |
55125 (7.3) |
122904 (-0.0) |
22172 (24.9) |
